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Determination of nitrate in whole tobacco: T-308 1 Scope of application 1.1 This method is applicable to determine nitrate in whole tobacco by an automated continuous flow colorimetric analyzer. 1.2 This method does not distinguish between nitrate and nitrite found in tobacco…

Determination of humectants in whole tobacco: T-304 1 Scope of application 1.1 Applicable to the determination of the amount of glycerol, propylene glycol, and triethylene glycol which may be added to whole tobacco as humectants. 1.2 This method is not designed to measure trace…

Determination of benzo[a]pyrene in whole tobacco: T-307 1 Scope of application 1.1 Applicable to the quantification of Benzo[a]pyrene (B[a]P) content in whole tobacco by high performance liquid chromatography (HPLC) via fluorescence detection. 2 Normative references 2.1 Health…

Determination of ammonia in whole tobacco: T-302 1 Scope of application 1.1 Applicable to the isolation and quantification of ammonia in whole tobacco by High Performance Liquid Chromatograpy (HPLC). 2 Normative references 2.1 Health Canada Official Method T-115. Determination of…

Neutral red uptake assay for mainstream tobacco smoke: T-502 1 Scope of applications 1.1 Applicable to the assessment of the cytotoxic potential of cigarette smoke. The method includes the preparation of Chinese Hamster Ovary (CHO) cells for assay, treatment with different…

In vitro micronucleus assay for mainstream tobacco smoke: T-503 1 Scope of applications 1.1 This document sets out how to test the total particulate matter from mainstream tobacco smoke on Chinese Hamster Ovary cells using the in vitro Micronucleus Assay. 2 Normative references…

Bacterial reverse mutation assay for mainstream tobacco smoke: T-501 1 Scope of applications 1.1 This document sets out how to test the total particulate matter (TPM) from mainstream tobacco smoke in the Bacterial Reverse Mutation Assay (Ames Assay) with the pre-incubation…

Determination of the pH of whole tobacco: T-310 1 Scope of application 1.1 This method is to be used to determine the pH of a 10% (w/v) suspension of tobacco in water by means of a pH meter. 2 Normative references 2.1 Health Canada Official Method T-115. Determination of Tar,…

Preparation of sample for testing of cigarettes, tobacco sticks, cigarette tobacco, cigars, little cigars, kreteks, bidis, leaf, pipe and smokeless tobacco: T-402 1 Scope of application 1.1 This method is applicable to the preparation of a homogeneous sample of tobacco product…

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